88'th update on fly longevity experiments

From: Doug Skrecky (oberon@vcn.bc.ca)
Date: Sat May 17 2003 - 21:52:37 MDT

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        This is the 88'th update of my fly longevity experiments. Average
    temperature was 19.9 C during this run. Estimated maximal longevity using
    the formula (363 - T*11.2) is 140 days.
        Here I test NAD and niacin at doses higher than those used in run #85,
    with null results.
        I also tried retesting rice protein, both new and old batches.
    Previous results in run #56 suggest rice protein is active in prolonging
    fly lifespan mostly in the absence of citric acid, which had since become
    a standard addition, since this acid keeps the fly food "fresh" and
    thereby increases fly lifespan. Unfortunately I added a little extra water
    in the current run, in the mistaken belief that the protein might exert a
    dehydrating effect. Instead the surface of the fly food became slightly
    sticky, which past experience has warned me is not a good thing for fly
    longevity. In the presence of citric acid, the rice protein was slightly
    detrimental, due I believe to this excess moisture. In the absence of
    citric acid, I once again notice a puzzling benefit. Although at acid pH,
    absorption of protein is known to be reduced, I suspect the real reason
    citric acid counters rice protein, is that the acid pH destroys the active
    ingrediant(s) in rice protein.

    Run #88 Percent Survival on Day
    supplement 37 43 48 53 60 67 72 77 82 89 95 101 106
    ___________________________________________________________________
    control 68 64 41 36 32 14 9 0 - - - - -
    NAD 10 mg 33 33 27 20 13 13 0 - - - - - -
    niacin 100 mg 32 26 26 26 11 5 0 - - - - - -
    niacin 200 mg 26 22 13 9 9 4 0 - - - - - -
    rice protein, old 1 tsp 47 41 29 18 12 6 0 - - - - - -
    rice protein, new 1 tsp 40 35 20 20 15 5 0 - - - - - -
    rice protein, new 2 tsp 30 25 25 25 15 5 0 - - - - - -
    -no citric acid control 36 36 29 21 7 7 0 - - - - - -
     -rice protein, old 1 tsp 50 50 45 40 35 25 20 20 15 5 5 5 0
     -rice protein, new 1 tsp 20 20 20 7 13 13 13 7 7 0 - - -
     -rice protein, new 2 tsp 31 15 15 8 8 8 8 8 0 - - - -



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