Authors
Zhang H. Osada K. Maebashi M. Ito M. Komai M. Furukawa Y.
Institution
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku
University, Sendai, Japan.
Title
A high biotin diet improves the impaired glucose tolerance
of long-term spontaneously hyperglycemic rats with non-insulin-dependent
diabetes mellitus.
Source
Journal of Nutritional Science & Vitaminology. 42(6):517-26, 1996 Dec.
Abstract
The Otsuka Long-Evans Tokushima Fatty (OLETF) rat, serving as a spontaneously
diabetic model with non-insulin-dependent diabetes mellitus
(NIDDM), exhibits impaired glucose tolerance (IGT) at about 16 weeks of age.
In this study, we investigated whether or not biotin, a
water-soluble vitamin, improved the IGT of OLETF rats. To this end, we
administered diets containing one of three levels of biotin,
a high-biotin diet (BH), a normal-biotin
diet (BN) and a basal-biotin diet (BB), to OLETF rats up to
24 weeks of age. An oral glucose tolerance test (OGTT) was performed four
times between 13 and 22 weeks of age. The administration of a BH corrected
the IGT of OLETF rats. Upon further investigation, we found that insulin
secretion in the OLETF-BH rats was decreased to a significant extent,
signaling that the hyperinsulinemia typical to the OLETF-BH rats had clearly
improved. Body weights were significantly lower in the OLETF-BH group than in
the other OLETF groups, even though the OLETF-BH rats showed a significantly
higher average daily food intake. The body weight gain of the OLETF-BH rats
followed the same tendency as the control-LETO (Long Evans Tokushima Otsuka)
rats (LETO-BB and LETO-BN). These results demonstrate that a high-level
biotin diet can improve the glucose handicap in NIDDM rats.