Study: Gene Therapy Restores Cells

J. R. Molloy (
Tue, 14 Sep 1999 09:23:33 -0700

Study: Gene Therapy Restores Cells


WASHINGTON (AP) _ Aged brains have been restored to youthful vigor in a gene therapy experiment with monkeys that may soon be tested in humans with Alzheimer's disease, researchers report. Scientists hope the treatment will reinvigorate thinking and memory.

``To our surprise, this technique nearly completely reversed''
the effects of aging on a group of key brain cells that had shrunk in elderly Rhesus monkeys, said Dr. Mark H. Tuszynski of the University of California, San Diego.

Tuszynski is senior author of a study appearing Tuesday in the Proceedings of the National Academy of Sciences.

The studies reinforce a new understanding of how the brain ages and suggest that neurons in the older brain don't die at first, but go into shrunken atrophy, he said.

``We've all heard the dogma that we lose 10,000 neurons a day
after the age of 20,'' said Tuszynski. ``Well, that is false. That doesn't happen.''

An actual count of the cells in the cortex, a key area in the thinking part of the brain, shows that very few cells are lost with age, he said.

Instead, he said, his team found that it was control neurons in another part of the brain, called the basal forebrain, that were most dramatically affected by aging. These cells, Tuszynski said, had shrunk in size and had stopped making some regulatory chemicals, a change that seriously affects the thinking cortex.

``These cells are like the air traffic controllers of the
brain,'' said the researcher. ``They are on the ground, deeper in the brain, controlling the activities of cells up there in the cortex. They control the flow of information in the cortex.''

The researchers found that about 40 percent of the basal forebrain cells could not be detected in old monkeys, and the other 60 percent had shrunk in size by 10 percent.

But the cells were not dead, Tuszynski said. By inserting genes for nerve growth factor, or NGF, into the brain, he said, the cells were revived and restored to nearly full vigor.

``We restored the number of cells we could detect to about 92
percent of normal for a young monkey and size of the cells was restored to within 3 percent,'' he said.

It isn't known yet if the restored cells also reinvigorated the old monkeys' thinking and memory, but that is now being tested in another group of old monkeys, he said.

But the therapy is so promising that the researchers applied in June to the Food and Drug Administration to test the gene therapy technique in humans with Alzheimer's disease.

If the FDA gives its approval, NGF genes will be injected into the brains of Alzheimer's patients to see if they will restore some cognitive powers gradually destroyed by the disease, he said.

Alzheimer's disease does not occur in animals exactly how it does in humans, said Tuszynski, so the only way to test the gene therapy technique is in human patients. The early trials, called Phase I, would involve only a small number to determine safety. It could be years before the technique's full value is proven, said Tuszynski.

Dr. Bradley Wise of the National Institute of Aging said the study is important because it suggests that ``the decline in the numbers and size of neurons with aging may be reversible.''

``A lot of studies have been done in rats in this area, but this
is a step forward because it used primates (Rhesus monkeys),'' said Wise. However, he cautioned that ``a lot of work will have to be done,'' including determining how long the gene treatment lasts, before the technique could be used routinely in humans.

In their experiment, the University of California, San Diego researchers used eight monkeys with an average age of 23, the monkey equivalent of the late 60s to 70s in humans.

Skin cells were taken from each of the monkeys. Into these cells, the researchers inserted a gene that makes human nerve growth factor, an essential chemical found in the brain. The modified cells were then injected into the forebrain of four of the monkeys. Four others, acting as controls, got injections of skin cells without the NGF gene.

     Once in the brain, the modified cells began making NGF.
     After three months, the researchers examined the brains of the
eight monkeys. The control monkeys showed a brain cell loss expected for animals their age. But the brains of the monkeys with the NGF genes injections had an almost youthful appearance, said Tuszynski.