From: Automatic digest processor <spamless_LISTSERV@VM.EGE.EDU.TR> To: Recipients of LONGEVITY-DIGEST digests <spamless_LONGEVITY-DIGEST@VM.EGE.EDU.TR> Subject: LONGEVITY-DIGEST Digest - 21 Jul 1998 to 27 Jul 1998
5. Anti-aging hormesis effects
Date: Mon, 27 Jul 1998 12:40:47 +0200
From: Suresh Rattan
Subject: Anti-aging hormesis effects
Here is the abstract of my recent publication on the anti-aging hormesis effects of heat shock on human skin fibroblasts. Previously, anti-aging effects of heat shock have been reported by others for Drosophila and nematodes. Since the present experiments with human cells in culture took much longer time than those for insects and worms, these results are being published just now.
=46or full manuscript see:
Biochemistry and Molecular Biology International; vol 45; (issue 4) pp 753-759, 1998.
REPEATED MILD HEAT SHOCK DELAYS AGEING IN CULTURED HUMAN SKIN FIBROBLASTS
Suresh I. S. Rattan
Laboratory of Cellular Ageing, Danish Centre for Molecular Gerontology, Institute of Molecular and Structural Biology, University of Aarhus, Gustav Wieds Vej, DK-8000 Aarhus - C, Denmark
Summary: The effects of repetitive mild heat shock (30 min, 41=B0 C) on growth and various cellular and biochemical characteristics of human skin fibroblasts undergoing ageing in vitro were analysed. Human skin cells not only tolerated more than 30 repeated heat shocks throughout their replicative lifespan, but also maintained several characteristics of young cells until late in life. Whereas the growth rates, population doubling rates, and cumulative population doubling levels achieved in vitro remained unaffected, age-related changes in cellular morphology, cell size, cytoskeletal organisation, autofluorescence and neutral b-galactosidase activity were significantly slowed down by repeated mild heat shock. These hormesis-like effects of stress-induced defence processes can be useful to elucidate the role of maintenance and repair mechanisms in ageing.
Key words: anti-ageing, homeostasis, hormesis, gerontogenes
Dr. Suresh I. S. Rattan, PhD; DSc
Laboratory of Cellular Ageing
University of Aarhus
DK-8000 Aarhus - C